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Isolation, fractionation and partial characterization of the tegumental surface from protoscoleces of the hydatid organism, Echinococcus granulosus | Parasitology | Cambridge Core
The effectiveness of each method and the sexual morality of subsequent microthrix-enriched fractions obtained by derived function natural process were evaluated by electron microscopy, by the amount of protein released and by the degree of enrichment of aspect plasma membrane marker enzymes. care in glucoside for 10 min produced the purest microtriche preparation, but in low yield; freeze/thawing, birthing in Triton X-100 for 10 mm or in glucoside for 20 min produced fractions containing significant amounts of relatively pure microtriches, but modest homogenization was a poor method for surface disruption and subsequent isolation of microtriches. Phosphodiesterase, adenosine triphosphatase (total and ouabain-inhibited), leucine aminopeptidase and glutamyltransferase were active in the protoscoleces but none were enriched in any of the microthrix fractions.